Screening of 200 Core SNPs and the Construction of a Systematic SNP-DNA Standard Fingerprint Database with More Than 20,000 Maize Varieties

To strengthen the management of maize varieties and protection intellectual property rights to new varieties, we constructed a comprehensive single nucleotide polymorphism (SNP)-DNA standard fingerprint database 20,075 materials covering nationally provincially approved hybrid lines, hybridized combinations, inbred lines. The was based on 200 core SNPs selected from 60 K distributed in intragenic regions, including 106 (53.0%) located exons. Average minor allele frequencies (MAF) 6755 hybrids, 7837 3478 lines were 0.385, 0.350, 0.378, respectively, with corresponding average information content (PIC) values 0.354, 0.335, 0.351. Heterozygous genotype averaged 0.48, 0.47, 0.012, respectively. number different loci three groups ranged one up 164, 160, 140, percentage within 5% (the difference is less than 10) accounted for 0.013%, 0.011%, 0.030% among pairwise comparisons samples combinations Genetic distances between highly correlated those obtained using SNPs, correlation coefficient 0.82 0.87 SNP-DNA established this study can play an important role variety authentication, purity determination rights, thereby providing reliable, data support use seed industry.